FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007358436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007358436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35797569
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTTAAGATCTCGTATG10612492.9645839917230132TruSeq Adapter, Index 11 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTTAAGATCTCGTAT8381392.3413293790983407TruSeq Adapter, Index 11 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTTAAGAACTCGTATG447380.12497496687554398TruSeq Adapter, Index 11 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)