FastQCFastQC Report
Tue 14 May 2024
EGAF00007358459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007358459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59364507
Total Bases7.4 Gbp
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCATCCTTGTGTAGATCT10862301.829763363485862Illumina Single End Sequencing Primer (100% over 32bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCATCCTTGTGTAGATCTC4266960.7187729193135555Illumina Single End Sequencing Primer (100% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)