FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007358462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007358462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46845142
Sequences flagged as poor quality0
Sequence length125-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATGAGAATCTCGTAT16174283.452712343149691TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATGAGAATCTCGTATG959280.20477683683827874TruSeq Adapter, Index 7 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATGAGAAACTCGTAT601740.12845302080629833TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATGAGAAGCTCGTAT492200.10506959291531233TruSeq Adapter, Index 7 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)