FastQCFastQC Report
Mon 13 May 2024
EGAF00007358463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007358463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46845142
Total Bases6.1 Gbp
Sequences flagged as poor quality0
Sequence length125-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATGCCTCGTGTAGATCT17097573.6498064196283146Illumina Single End PCR Primer 1 (96% over 33bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATGCCTCGTGTAGATCTC1109840.23691677570323086Illumina Single End PCR Primer 1 (96% over 32bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)