FastQCFastQC Report
Sat 18 Mar 2023
EGAF00007358465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007358465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57220155
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGCACCTGTGTAGATCT5762591.0070909454893997Illumina Single End PCR Primer 1 (96% over 33bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGCACCTGTGTAGATCTC1948540.34053385559686095Illumina Single End PCR Primer 1 (96% over 32bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)