FastQCFastQC Report
Mon 13 May 2024
EGAF00007358471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007358471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60944359
Total Bases8.1 Gbp
Sequences flagged as poor quality0
Sequence length101-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGACAACGTGTAGATCT7051861.1570980671074085Illumina Single End PCR Primer 1 (97% over 34bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGACAACGTGTAGATCTC3417560.5607672401640978Illumina Single End PCR Primer 1 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)