FastQCFastQC Report
Sat 18 Mar 2023
EGAF00007358483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007358483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70174745
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGCTATGTGTGTAGATCT10162681.4481962136093263Illumina Single End PCR Primer 1 (97% over 34bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGCTATGTGTGTAGATCTC6383200.9096149904071614Illumina Single End PCR Primer 1 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)