FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007358486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007358486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39184057
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCAGGATCTCGTATG8331622.126278042112893TruSeq Adapter, Index 14 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCAGGATCTCGTAT3766550.9612455392253028TruSeq Adapter, Index 14 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)