FastQCFastQC Report
Mon 13 May 2024
EGAF00007358499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007358499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46676365
Total Bases5.8 Gbp
Sequences flagged as poor quality0
Sequence length125
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGCGAGACGTGTAGATCT5321121.1400030829307295Illumina Single End PCR Primer 1 (97% over 34bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGCGAGACGTGTAGATCTC1256710.26923904635675894Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)