FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007358548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007358548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43400666
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCGAGGATCTCGTAT17540344.041490976198384TruSeq Adapter, Index 10 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCGAGGATCTCGTATG6846581.5775287872310533TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCGAGGAGCTCGTAT1015870.23406783665485686TruSeq Adapter, Index 10 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCGAGGAACTCGTATG857940.19767899414262444TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCGAGGAACTCGTAT850660.1960016005284343TruSeq Adapter, Index 10 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCGAGGAGCTCGTATG694340.15998372006549394TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCGAGGACCTCGTAT497030.1145212840743043TruSeq Adapter, Index 10 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)