FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007358572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007358572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57710444
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATATCTCGTAT812439314.077855647757623TruSeq Adapter, Index 13 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATATCTCGTATG5569420.9650627536326006TruSeq Adapter, Index 13 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATAGCTCGTAT2197010.3806953902486004TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATAACTCGTAT2124030.36804949897803596TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATACCTCGTAT1319380.22862066353189037TruSeq Adapter, Index 13 (97% over 37bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATATCTCGTAT743980.12891600695361138TruSeq Adapter, Index 13 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)