FastQCFastQC Report
Sat 18 Mar 2023
EGAF00007358582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007358582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236298473
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGGCATGATCTCGTAT138348025.854799577989656TruSeq Adapter, Index 15 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGGCATGATCTCGTATG13899250.5882073558723335TruSeq Adapter, Index 15 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGGCATGAGCTCGTAT5916480.25038164338878316TruSeq Adapter, Index 15 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGGCATGAACTCGTAT5142440.21762476645373835TruSeq Adapter, Index 15 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGGCATGACCTCGTAT2969060.12564871716289086TruSeq Adapter, Index 15 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)