FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007358586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007358586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49153856
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATGCAATCTCGTAT503608110.245546147997016TruSeq Adapter, Index 6 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATGCAATCTCGTATG4846850.9860569229807727TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATGCAAGCTCGTAT1586750.3228129243817616TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATGCAAACTCGTAT1493010.30374219267762026TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATGCAACCTCGTAT872530.17750998009189756TruSeq Adapter, Index 6 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATGCAAACTCGTATG507900.1033286177995883TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)