FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007358602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007358602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17126666
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTACTCATAATCTCGTATG9703405.665667795471693TruSeq Adapter, Index 7 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACTCATAATCTCGTAT8665705.059770535608039TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTACTCATAAACTCGTATG703510.41076879761653556TruSeq Adapter, Index 7 (97% over 34bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTACTCATAAGCTCGTATG306160.17876217122468555TruSeq Adapter, Index 7 (97% over 34bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTACTCATAACCTCGTATG275520.1608719408669498TruSeq Adapter, Index 7 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACTCATAAGCTCGTAT210760.12305956103774081TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACTCATAAACTCGTAT203870.11903659474646144TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACTCATAACCTCGTAT201500.11765278776382981TruSeq Adapter, Index 7 (97% over 35bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACTACTCATAATCTCGTAT173290.10118139747689364TruSeq Adapter, Index 10 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)