FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007358603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007358603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17126666
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCACAGGGTGTAGATCTC9550105.576158255202735Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCACAGGGTGTAGATCT8392964.900521794492869Illumina Single End PCR Primer 1 (96% over 32bp)
NTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCACAGGGTGTAGATCTC182780.1067224642554482Illumina Single End PCR Primer 1 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)