FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007358616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007358616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50890091
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATTCTGCATCTCGTAT19872963.905074565498419TruSeq Adapter, Index 21 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATTCTGCATCTCGTATG1554510.30546418162231226TruSeq Adapter, Index 21 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATTCTGCAGCTCGTAT666370.13094297669854824TruSeq Adapter, Index 21 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATTCTGCAACTCGTAT605990.11907819146953383TruSeq Adapter, Index 21 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)