FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007358618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007358618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18096903
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACCGCGATCTCGTATG12043486.6549950563364355TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACCGCGATCTCGTAT7886914.35815454169147TruSeq Adapter, Index 9 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACCGCGAACTCGTATG969590.5357767569401239TruSeq Adapter, Index 9 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACCGCGAGCTCGTATG658040.3636202282788386TruSeq Adapter, Index 9 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACCGCGACCTCGTATG479260.2648298440898976TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACCGCGAGCTCGTAT299530.16551450820065733TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACCGCGAACTCGTAT271910.15025222824037904TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACCGCGACCTCGTAT256820.14191378491667883TruSeq Adapter, Index 9 (97% over 37bp)
NTCGGAAGAGCACACGTCTGAACTCCAGTCACGAACCGCGATCTCGTATG212300.11731289049844607TruSeq Adapter, Index 9 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)