FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007358619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007358619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18096903
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGGTCAGTGTAGATCTC11955236.6062298062823235Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGGTCAGTGTAGATCT7907724.36965374683171Illumina Single End PCR Primer 1 (96% over 32bp)
NTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGGTCAGTGTAGATCTC228640.12634205974359258Illumina Single End PCR Primer 1 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)