FastQCFastQC Report
Wed 16 Nov 2022
EGAF00007405494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007405494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355033278
Sequences flagged as poor quality0
Sequence length100
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTCAAGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTGT3682432610.372077290174472No Hit
TCCATAACAGCTTTCAGTTGCTCTTTTGTTGCCTTGGGCTTGTGTTTCAC100625362.83425149796803No Hit
TCACACCATCCTCACGGTAGTCCAATAGAGCAACCATGCCATCTGGATTC53577561.5090855792960343No Hit
GGCATGATCTCGGCTCACTGCAACCTCCGCCTCCTGAGGTCAAGTGATTC24379780.6866899952967226No Hit
AATGGCCAATTGAAACAAACAGTTCTGAGACCGTTCTTCCACCACTGATT20004960.563467180110367No Hit
TTGCATCCCACCTCTGAGACGGAGCACCAGGTGCAGGGTGGACTCTTTCT18914300.5327472429218311No Hit
TGCGCAGGCGCTGGGTGGACTGCTTCCAGGTGTCATATTGGAAGAACTTG18546360.5223837073661585No Hit
CTTCTGAAACACAGTCCATCCATTTCCAGACCCATCGATTTCACAGTAGA17622980.4963754411776577No Hit
AAGACAATGGTCTGGACACTTCACTGGGTGGCAGGCTGTGTTCCAAGTTC15753880.4437296720111967No Hit
AGTCCATGCTGTGCCCACCACACTTAGGACCAGGCAGACGATCCTCATGG11268420.31739052923371314No Hit
GAATAATTCTGACGAATCTGGGAGCTCATGGTTGGTTGGCAAGAAGGAGC10344900.291378319752888No Hit
AGCCACCAGAGCTGCTTCCTCAGTTTCTGGATCATCGCAGTTCGGTTGTC9365420.26378991999730234No Hit
ATATTTCTGTTGCCACATTGCCCTTCCTCGTCTTGACGGTAAAGTGAGTG9172000.25834197998757735No Hit
ACAGCTTCTTACAGTGCTGGATGTTAAACATGCCTAAACGCTTCATCATA8374860.23588943682062388No Hit
ACTCCTCGAACACGTGGCCCCGCTTCCTGTTCAAAACCCCGTAGATGCCA7837420.2207517009152027No Hit
CTGGTGATGATCAGCTTCTGCCCCTTCACTTCCATGCTGGCCTCTGGCTT6959760.19603120133431548No Hit
TGCCTCTGCTTCAACAATGTGCAACTCATCCTTTGCACCAGCCCCTAAAC6844700.1927903783712354No Hit
GGGTCCAGAGATTGATGGTGCTCCTTCACCCTTTCAGACACATACTCTTT6315500.17788473338547153No Hit
CCCGCCTCTTCACGGGTAGGTCAATTTCACTGGTTGAAAGTAAGAGACAG6218780.17516048171687162No Hit
TTCTTTGATGTGAAAGGGGCAGCACAGTCATTTAAACTTGATCCAACCTC6195260.17449800860639325No Hit
CGGCACAGGAGCAGTTGGGGTCCATTGCAACCCGAGGCGAGACTAGAGTT6058980.17065949519244786No Hit
AGGGACGCCATCTCTGCCAGGTTCTCCAGCAGCTCCTCTGGGCCCTGCAG5841420.16453161892052273No Hit
GTGTGACCACACTTCTTCTTGCGGCAGTTGACAGCACGAGGGTGAAGGCG5740300.16168343520744555No Hit
GGTGTCTGGGGCGCCCGACGATGGAAGGAAACACGGCTCGGGGAGCGTCG5640260.15886567117801279No Hit
GCTTCCTGCGTCTCAAAGTGTACAAATCCATAGCCCTTGGAACCATTTTC5496140.15480633339390795No Hit
GCTTGCTGAAGGTGGAGGTCACGCTGTCCCAGTTGTCAAGGAGCTTTAGG5039100.141933173937571No Hit
GGCGAGCTGAAGAGGCGGCTAGAGTCGGGACAGGTTGCACGCGGGTTCGC4721520.132988096963688No Hit
TCCAAATCCCAGAACTCAGAGAACTTGTCCTTAACGGTGCTCCAGTAGTC4688180.1320490300630354No Hit
CCCATCTGTGTTGGTTGCAACATTTCCAAATCCCTGTTTATATGGATCCC4630020.1304108737660361No Hit
TAGGAAATTTCACTCCGCTTCTCCTGCTCAATGGTCTCTTTGGTCGGCAG4582380.12906902772083242No Hit
CACGAAGCCACAAAGCTACTTGGGTTCCTCCTTCTTCTCGTTTGCCTTTT4510200.12703597886393062No Hit
TGGACAGTAACAGTGACTGGAACATCCCCTTGCGCGTCGTGGGCCTCCAG4425300.12464465373299458No Hit
TCATCCCTCAGCCAGAGATTAGTCTACTTCTTCCATGCGTGATGTGTCGT4350360.12253386568455704No Hit
TTGGCCTCAGGAAGTGGCAGCACCTCACCTTTTCTTAGAGGAGGGAGTCG4228200.11909306146788866No Hit
AGTGTTCTGTGGGACAGTCTGGTGTTTCACAAAGGCCACATCTCCCTTCT4127180.11624769439218596No Hit
TGTGACTCGTGCAGCCATCGACAGTGACGCTGTAGGTGAAGCGGCTGTTG4029200.11348795309266756No Hit
GGAGCAGGTTTCCTCGATTATCCTTCTTGCTCCAGGTGTCTCCAATTCTA3843480.1082568941607778No Hit
TTCCCCGCGGCCTTCGCTTTTAGCTCCTCGAGTTTCTTCTGCTCCTCTTT3827700.10781242878308438No Hit
CCCACGGCTATGGGGAAATTAGCCCGAGGCTTAGCTTTCATTATCACTGT3816420.1074947120872427No Hit
CCAGGCTCCCCAGGGCGACCAGCATCTCCATTAGGTCCTCGGACTCCAGC3785800.10663225772317601No Hit
TGTAGGATTTTTATTGGTGGCACCTGGGGCCACATGGAGGGAGTCCTCAG3736900.10525492204705386No Hit
CTCTTGCTGGGGCTGGTGGTCCAGGGGTCTTACTCCTTGGAGGCCATGTG3685580.1038094237464692No Hit
ATGACCTCACAGCTCAAGTACACCTGGGCACCAGTGACATTCCAGATGTC3634360.10236674208325902No Hit
CTGGCAGCCCACTCCTCATCCTTCTCCACAACAGAGCTCCTCCTACAGCC3588900.10108629873281907No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)