FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007438275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007438275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59612296
Sequences flagged as poor quality0
Sequence length35-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT8579351.4391913373039684No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC4056410.6804653187657794No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG3541720.5941257488220215No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT3380090.5670122150638184No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC3135290.5259468617011497No Hit
GTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT2931330.4917324439239851No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT2835650.47568206398223617No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT2652840.445015571955155No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTT2201310.369271131579968No Hit
CTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTA2131150.35750174762602666No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG2040950.34237064111739635No Hit
GGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT1887060.3165554972081599No Hit
CTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGC1797150.30147303838120915No Hit
CTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGG1700090.2851911625749158No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG1694770.2842987292420342No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG1667430.2797124271140303No Hit
CTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTC1584900.2658679679105129No Hit
GGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAA1559200.26155677680993866No Hit
GTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTC1517560.25457164072324945No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT1486580.2493747263148529No Hit
CTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGG1439370.24145521923866178No Hit
GGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTT1431880.2401987670463154No Hit
GTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATT1324180.22213202457425896No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT1317440.22100138535177372No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG1211870.2032919517141229No Hit
GCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGG1162440.19500003824714285No Hit
CACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACA1158430.19432735823495206No Hit
GCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAA1147500.19249384388750937No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC1137350.19079117502872225No Hit
ATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGT1120990.1880467747794851No Hit
GTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATA995090.16692697090546554No Hit
CCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTT969610.1626526849427172No Hit
AGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCA964640.1618189643291042No Hit
GCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATT923440.1549076385180668No Hit
GGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTG884380.14835529904769984No Hit
GGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCA881370.14785036966199053No Hit
AGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTT869460.145852459700596No Hit
CTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGC868030.14561257630472746No Hit
GTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCA806150.1352321675380529No Hit
GTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTA799230.13407133320280099No Hit
GGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATT798580.133962295295588No Hit
GGCAATGCTTGTGGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTA781930.1311692473646712No Hit
CGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGG775960.13016777612457672No Hit
TTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCA769160.1290270718645026No Hit
CTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGG757900.127138199810321No Hit
CTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAG732910.12294611165454858No Hit
CTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCA711120.11929082550351693No Hit
CCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCAC699210.11729291554212239No Hit
CCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATA667260.11193328302603878No Hit
CGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCA657410.11028093935519612No Hit
TCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCAC646250.10840884236366269No Hit
CTCCACTTTTTCAATTCTCTCTCCATTCTTCAGTAAGTCAACTTCAATGT638860.10716916523396448No Hit
CCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCTG608930.1021483889833735No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)