FastQCFastQC Report
Sat 17 Dec 2022
EGAF00007438277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007438277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28076648
Sequences flagged as poor quality0
Sequence length35-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC4155321.4799914861631631No Hit
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT3898291.3884456577580058No Hit
GTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT3367551.1994131208255345No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT3016211.074277100314824No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT2888121.0286555574582836No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC2760500.9832014135020677No Hit
GTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTC2589420.9222682137839247No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG2142260.7630041876793839No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG2032730.7239931205462988No Hit
GGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTT1869050.6656955630885852No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT1656260.5899066013863193No Hit
GTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG1570010.5591871223373959No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG1527160.5439253289780176No Hit
GTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATT1439770.5127998185538387No Hit
GGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAA1270380.4524685425411182No Hit
GCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAA1243140.4427665296797538No Hit
GGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT1240240.4417336428479639No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT1215170.43280451427107675No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC1208520.4304359979154207No Hit
CTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTA1193940.42524307032662867No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT1005450.3581089879390161No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG974830.34720312766680694No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTT889510.3168148847398023No Hit
AGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCA877870.3126690906977215No Hit
CCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCTG871530.3104109863827049No Hit
CTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGC847760.30194487604075815No Hit
ATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGT750320.26723987849261777No Hit
GGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATT702340.25015094394459053No Hit
GTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCA699490.2491358655064522No Hit
GTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATA684360.24374704558749322No Hit
GCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGG674470.24022454532321666No Hit
CTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTC661390.23556586954397118No Hit
CTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGG652450.2323817287590741No Hit
GGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTG621550.22137614148241627No Hit
CTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGG583480.2078168305561262No Hit
GTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTA581860.20723983860181602No Hit
GCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATG580460.2067412035795726No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG573410.20423022007470407No Hit
CCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTT567110.2019863624746088No Hit
GTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA557790.19866687789795992No Hit
CACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACA541710.19293969849962148No Hit
GGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCA537570.19146516350527315No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG530090.1888010278150013No Hit
CGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGG524420.18678155597491553No Hit
GGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCG503990.17950504632889225No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA496340.17678036210020512No Hit
CTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAG489520.17435129720613374No Hit
TCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCAC484720.17264169141558494No Hit
AATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGC446550.15904676370199178No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG437340.15576645759137628No Hit
GTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCTGGG433920.1545483634656103No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG421180.15001078476319538No Hit
GGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAAT416500.1483439191174103No Hit
AGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTT416440.14832254904502845No Hit
GCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATT414480.1476244600138877No Hit
GTGGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCT401840.1431224980987759No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC398990.14210741966063756No Hit
GCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCTGGGCTCT395840.14098549086058992No Hit
CGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCA395640.14091425728598372No Hit
CCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTTG391790.13954301097481436No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT360200.12829166786576518No Hit
CCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCAC351710.12526780262373202No Hit
CATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGG348430.12409957200019033No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC346710.12348696325857703No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTT324440.11555510472617671No Hit
ACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTG285450.10166811935669814No Hit
CTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGC281070.10010810407282238No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)