FastQCFastQC Report
Sat 17 Dec 2022
EGAF00007438278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007438278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28076648
Sequences flagged as poor quality0
Sequence length35-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT4033151.436478457114966No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA2514380.8955413765916785No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2319800.8262382318573072No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG2032030.7237438030351772No Hit
CCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTG1931400.6879026299720679No Hit
AATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTG1688760.6014820572598267No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCT1536520.5472590602695877No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC1368790.48751902292609856No Hit
GTTTGTTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTT1362800.48538557736664295No Hit
GAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATT1205200.42925352057695776No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT1185060.4220802996141135No Hit
CACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCA1054630.3756253239346805No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT1049480.3737910593885709No Hit
GTTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTT960960.34226307926786703No Hit
GCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCT904140.3220256207222457No Hit
GGTTTGTTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCT864950.308067401778161No Hit
CGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGG841480.29970814179812344No Hit
TGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAAT802870.28595650022039665No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG786640.2801758956411036No Hit
GGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGT759100.27036703241782994No Hit
GGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCG663770.23641354908178497No Hit
GGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA660620.2352916202817373No Hit
ATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAA649880.23146637732538444No Hit
ATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCC618780.22038955647412042No Hit
CAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAG594720.21182015744899463No Hit
TATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAA551740.19651206226612236No Hit
CAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGT538940.1919531134913256No Hit
GTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCT535950.19088817155096294No Hit
AAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGT523280.1863755245996602No Hit
CCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAAGTAGGGACCTG516010.18378618416272485No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT512700.18260726850299222No Hit
GTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGA497250.17710447486466332No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA476750.1698030334675279No Hit
ATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTC474360.1689517922509838No Hit
GGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGG473520.16865261123763778No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACT466820.16626628648833008No Hit
CCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTC463940.16524052301400083No Hit
GTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAG451560.16083116474587708No Hit
CATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACT448490.1597377293756719No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC437740.15590892474058868No Hit
GGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACC434000.15457685689545278No Hit
GTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATA431830.1538039726109755No Hit
GCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAA431500.15368643721287528No Hit
CACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCC426400.1518699810604172No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAAGTAGGGACCT421970.15029215738288987No Hit
CTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTT401420.14297290759210288No Hit
AGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGA398680.14199700761999795No Hit
CACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGC374210.13328157976692942No Hit
GGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCT357050.12716973906571752No Hit
CAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCTTAGCTGTGCTCGCGC354940.12641822485362214No Hit
AATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTT345520.12306312348967013No Hit
AAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTG344470.12268914722298757No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAG343570.1223685961372597No Hit
CCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT339530.12092967793021446No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT339390.12087981442799013No Hit
AACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGG333820.11889595937520747No Hit
GGTACTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAAC332870.11855759989582801No Hit
CTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAACCATGTC324600.11561209158586166No Hit
GGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA322370.11481783722900256No Hit
GACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT320850.11427646206199545No Hit
GGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAG314950.11217507161111256No Hit
CACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTT311190.11083588040851601No Hit
GAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTT310160.11046902749929408No Hit
GTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCAC304480.10844599398047801No Hit
GTACTGTTTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTT301930.10753776590424896No Hit
ATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTC298210.10621282141657366No Hit
AGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCAT295600.10528322326796276No Hit
CTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAA285440.10166455767796784No Hit
TAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCC285030.1015185288500251No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)