FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007438347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007438347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2558245
Sequences flagged as poor quality0
Sequence length88-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1630516.373549054136722RNA PCR Primer, Index 1 (100% over 28bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1167014.561760112889892RNA PCR Primer, Index 2 (100% over 34bp)
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA866373.386579471473608RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG784823.0678062499877847RNA PCR Primer, Index 1 (100% over 29bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA706332.7609943535509696RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTATCTCGTATGC582402.2765606890661374RNA PCR Primer, Index 38 (100% over 50bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTA529902.0713418769508003RNA PCR Primer, Index 2 (97% over 36bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC525182.052891728509193RNA PCR Primer, Index 1 (100% over 27bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT411701.609306379959699RNA PCR Primer, Index 1 (97% over 35bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA407291.5920679997420104RNA PCR Primer, Index 1 (100% over 28bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC320221.2517174860109175RNA PCR Primer, Index 1 (100% over 33bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA307761.2030122212688776RNA PCR Primer, Index 1 (100% over 32bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTATCGCGTATGC242110.9463909828808421RNA PCR Primer, Index 38 (98% over 50bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC182750.7143569126491013RNA PCR Primer, Index 1 (100% over 27bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA136600.5339598044753336RNA PCR Primer, Index 1 (100% over 23bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA125600.49096157717497735RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA121450.4747395186934793RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA110830.43322668469986264No Hit
TGCGGAAGGATCATTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC93890.3670094146573139RNA PCR Primer, Index 2 (100% over 34bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT80750.315646077682161RNA PCR Primer, Index 1 (100% over 30bp)
AGTCCGCGTGCTGATAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT78680.3075545930901849RNA PCR Primer, Index 1 (100% over 30bp)
AAGTCCGCGTGCTGATAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG73830.2885962837804823RNA PCR Primer, Index 1 (100% over 29bp)
TGATACGGATGTTATACGCAGCTGGAATTCTCGGGTGCCAAGGAACTCCA73310.2865636403081019RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT62250.24333087722247085RNA PCR Primer, Index 1 (100% over 30bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA59870.2340276244065756RNA PCR Primer, Index 1 (100% over 32bp)
TAGCATATCGAGCCTGAGAACATGGAATTCTCGGGTGCCAAGGAACTCCA59340.23195589163664934RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG54470.21291940373185525No Hit
GTCCGCGTGCTGATAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC50290.19658007735771985RNA PCR Primer, Index 1 (100% over 31bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC49560.19372655863687802RNA PCR Primer, Index 1 (100% over 27bp)
CTGCGGAAGGATCATTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC48520.18966127169211705RNA PCR Primer, Index 1 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC48310.18884039644365572No Hit
TGAGGGGCAGAGAGCGAGACTTGGAATTCTCGGGTGCCAAGGAACTCCAG48040.1877849854099197RNA PCR Primer, Index 1 (100% over 29bp)
GGAAGTCCGCGTGCTGATAGCTTTGGAATTCTCGGGTGCCAAGGAACTCC44130.1725010700695203RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC44000.17199290920142518RNA PCR Primer, Index 1 (100% over 26bp)
CCTGGAACTTAGGACGTGAATCTGGAATTCTCGGGTGCCAAGGAACTCCA42110.16460503196527307RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGGTGGAATTCTCGGGTGCCAAGGAACTCCAG41660.16284601357571304RNA PCR Primer, Index 1 (100% over 29bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC39020.15252643902362753No Hit
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT38360.14994654538560614RNA PCR Primer, Index 1 (100% over 25bp)
GAAGTCCGCGTGCTGATAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA38090.14889113435187012RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG37130.1451385617874754RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAATGGAATTCTCGGGTGCCAAGGAACTCCA36000.14072147116480244RNA PCR Primer, Index 1 (100% over 28bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCA35050.1370079878979535RNA PCR Primer, Index 1 (100% over 28bp)
TTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC34700.13563986248385124RNA PCR Primer, Index 1 (100% over 31bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC33550.1311445932660867RNA PCR Primer, Index 1 (100% over 27bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAG33460.1307927895881747RNA PCR Primer, Index 3 (97% over 37bp)
AATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG32830.12833016384279067RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC31140.12172407255755412RNA PCR Primer, Index 1 (100% over 26bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA30850.12059048292872653RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG28080.1097627475085459RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG27800.1086682471772641No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT27380.10702649668034141No Hit
CCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAAC27340.10687013949015828RNA PCR Primer, Index 1 (100% over 24bp)
CCCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCTCGGGTGCCAAGGA26120.10210124518957332RNA PCR Primer, Index 1 (100% over 22bp)
CGATGCTATAGACTCTCACGTGATGTGGAATTCTCGGGTGCCAAGGAACT25820.10092856626319996RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)