FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007438354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007438354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15183485
Sequences flagged as poor quality0
Sequence length52-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA487008232.07486291849335RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG364595724.01264926991399RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC9430116.210767817796771RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC3194812.1041348544158343RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT3127992.0601265124574497RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2740691.805046733342181RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG1807181.190227408266284RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA1459710.9613800784207314RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1050110.6916132890439842RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTTGGAATTCTCGGGTGCCAAGGAACTCCAG891950.5874474799428457RNA PCR Primer, Index 1 (100% over 29bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC840260.5534039122111952RNA PCR Primer, Index 2 (100% over 34bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA749740.4937865055354551RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA700760.46152777178625326RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAATGGAATTCTCGGGTGCCAAGGAACTCCA652920.430019853808266RNA PCR Primer, Index 1 (100% over 28bp)
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG496760.32717126535838115RNA PCR Primer, Index 1 (100% over 29bp)
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA484690.31922183872806537RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC483300.3183063703754441RNA PCR Primer, Index 1 (100% over 27bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA433790.28569857315366004RNA PCR Primer, Index 1 (100% over 23bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC432930.285132168273621RNA PCR Primer, Index 1 (100% over 27bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC426550.28093023439612186No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA423550.2789544034192414RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA414530.2730137382820874RNA PCR Primer, Index 1 (100% over 28bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA389720.2566736161032859RNA PCR Primer, Index 1 (100% over 32bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA386280.2544079965831296RNA PCR Primer, Index 1 (100% over 32bp)
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG383170.2523597184704302RNA PCR Primer, Index 1 (100% over 29bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA372030.2450227994429474RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGTATGC371170.24445639456290832RNA PCR Primer, Index 37 (100% over 50bp)
CATTGCACTTGTCTCGGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT370700.24414684770986372RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC363060.23911506482207479RNA PCR Primer, Index 1 (100% over 26bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT356180.2345838257817622RNA PCR Primer, Index 1 (100% over 30bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG331190.2181251537443479RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG318030.20945784185909888RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA300800.19810998594854873RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGCATTGTACAGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC300600.19797826388342335RNA PCR Primer, Index 1 (100% over 31bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGG291660.19209028757231952RNA PCR Primer, Index 7 (97% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG282420.18600472816352767No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC278850.18365348930103992RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGATTGGAATTCTCGGGTGCCAAGGAACTCCA271920.17908931974444603RNA PCR Primer, Index 1 (100% over 28bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC266620.17559868501862386RNA PCR Primer, Index 1 (100% over 33bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA257810.16979632804985154RNA PCR Primer, Index 1 (100% over 28bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG256880.16918382044701857RNA PCR Primer, Index 1 (100% over 29bp)
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT251670.16575246065050284RNA PCR Primer, Index 1 (100% over 25bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG249280.1641783819722547RNA PCR Primer, Index 2 (100% over 35bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT247240.16283481690797602RNA PCR Primer, Index 1 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGAGATTGGAATTCTCGGGTGCCAAGGAACTC218130.14366267032897914RNA PCR Primer, Index 1 (100% over 26bp)
TACCCTGTAGAACCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG217390.1431752986880153RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC209700.13811058528394501RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGAATGGAATTCTCGGGTGCCAAGGAACTC208860.1375573526104185RNA PCR Primer, Index 1 (100% over 26bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA197070.12979233687127825RNA PCR Primer, Index 1 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA195670.1288702824154007RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTTTGGAATTCTCGGGTGCCAAGGAACTCCA185730.12232369577867006RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA174120.11467722989814261RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC171400.11288580981243766RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCGCGTATGC164430.10829529584281869RNA PCR Primer, Index 37 (98% over 50bp)
TAGCAGCACATAATGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT163590.10774206316929216RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA153230.10091886019579827RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)