FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007438358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007438358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11300937
Sequences flagged as poor quality0
Sequence length90-101
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA403015235.662104832546184RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG237499921.01594761567116RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC11157289.872880452302319RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC3556343.146942594229133RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1851311.6381915942014367RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1723661.5252363587196354RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG1066770.9439659737949163RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA1062190.9399132125061842RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA969880.8582297202435516RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAATGGAATTCTCGGGTGCCAAGGAACTCCA701060.6203556395367924RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTTGGAATTCTCGGGTGCCAAGGAACTCCAG555480.4915344630272694RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA507690.44924593420881825RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA451130.3991969869401095RNA PCR Primer, Index 1 (100% over 28bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG422570.3739247462400684RNA PCR Primer, Index 6 (100% over 34bp)
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA416750.3687747308032953RNA PCR Primer, Index 1 (100% over 28bp)
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG333670.2952587028845484RNA PCR Primer, Index 1 (100% over 29bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA326470.288887549766891RNA PCR Primer, Index 1 (100% over 28bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA318730.2820385601654093RNA PCR Primer, Index 1 (100% over 28bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA315040.27877334419260985RNA PCR Primer, Index 1 (100% over 32bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG308740.2731985852146597RNA PCR Primer, Index 1 (100% over 29bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT308520.27300391109162014RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG293840.2600138377906186RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC277490.24554601091927156RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA273640.24213921376607972RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATTGGAATTCTCGGGTGCCAAGGAACTCCA268330.2374404883418074RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAC264130.23372398235650726RNA PCR Primer, Index 6 (97% over 36bp)
CATTGCACTTGTCTCGGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT245820.21752178602535346RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC245470.21721207719324512RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGAATGGAATTCTCGGGTGCCAAGGAACTC224380.19854990785277363RNA PCR Primer, Index 1 (100% over 26bp)
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT222640.19701021251600642RNA PCR Primer, Index 1 (100% over 25bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG210620.18637392633902836RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGAACCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG210050.18586954338388048RNA PCR Primer, Index 1 (100% over 29bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC209610.1854801951378014RNA PCR Primer, Index 1 (100% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACATCTCGTATGC202540.1792240767292128RNA PCR Primer, Index 41 (100% over 50bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG197880.17510052485028454RNA PCR Primer, Index 1 (100% over 29bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA181410.16052651209364321RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC180430.15965932736373983RNA PCR Primer, Index 1 (100% over 26bp)
AGCAGCATTGTACAGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC178540.15798689967035479RNA PCR Primer, Index 1 (100% over 31bp)
TCCTGTACTGAGCTGCCCCGAGATTGGAATTCTCGGGTGCCAAGGAACTC173390.1534297554264748RNA PCR Primer, Index 1 (100% over 26bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA168920.1494743311992625RNA PCR Primer, Index 1 (100% over 23bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA166470.1473063693745041RNA PCR Primer, Index 1 (100% over 28bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA154930.13709482673870316RNA PCR Primer, Index 7 (97% over 35bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC142380.12598955290167532RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT134990.11945027213230194RNA PCR Primer, Index 1 (100% over 30bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA133130.11780439091024046RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTTTGGAATTCTCGGGTGCCAAGGAACTCCA127170.11253049194062403RNA PCR Primer, Index 1 (100% over 28bp)
CGATGCTATAGACTCTCACGTGATGTGGAATTCTCGGGTGCCAAGGAACT117650.10410641170727701RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)