FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007438412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007438412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49831184
Sequences flagged as poor quality0
Sequence length35-101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4300430.862999763361031No Hit
CTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT4035380.8098101783012019No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC2801240.5621459847311675No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT2728710.5475908419113622No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT2321400.4658528683564894No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT2114250.42428251353610225No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC1981050.39755226365883656No Hit
GTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT1925050.3863143207674937No Hit
GTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTC1465180.2940287350988891No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTT1442950.28956767312612924No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG1252460.25134060631591654No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG1185110.23782497321356041No Hit
GGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT1129550.22667532844493518No Hit
GGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTT1125500.22586258436082915No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG1123670.2254953444413442No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT1120080.22477491203098848No Hit
CTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGG809590.16246653902504102No Hit
GTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATT790890.15871386880953903No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG746450.1497957584150519No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT745030.1495107962917357No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT721260.1447406908894639No Hit
GGTGAATTCAGTGTAGTACAAGAGATAGAAAGACCAGTCCTTGCTGAAAG715430.14357074076345447No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG702140.14090373610227686No Hit
CTCCACTTTTTCAATTCTCTCTCCATTCTTCAGTAAGTCAACTTCAATGT669310.13431549208222707No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC661180.13268398358746603No Hit
GTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGAAG655360.13151604023697291No Hit
GTCATTTTTGTCAGTGGCCAGTTTGTGCAGTTCCAGTAGTGACTGATTCA631830.12679409744709258No Hit
GTGGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCT618580.12413511988798019No Hit
GCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAA614610.12333843000800462No Hit
CTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTC607060.12182331449318966No Hit
CTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGG593520.11910614044410422No Hit
CTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGC592120.11882519187182067No Hit
CCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTT590920.11858437880986332No Hit
GTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATA588770.11815292207385641No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC580520.11649733227289964No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT575800.11555013422920074No Hit
GTCCAGGCAGATTTTCCTCCCATCCTTCAGTGTGGCTATCACTTCGACTT569240.11423368949050057No Hit
GGCAATGCTTGTGGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTA567520.11388852410169503No Hit
GTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCT560440.11246772703614669No Hit
CTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTA548700.11011177257999731No Hit
GGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAA547890.10994922376317609No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCC536340.1076313980418366No Hit
GCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATT536180.10759928963357562No Hit
CACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACA528040.10596577436329829No Hit
GCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGG515180.10338506104932205No Hit
GTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCA513310.10300979402777186No Hit
CTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGTCT507520.10184787100382764No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA505270.10139634651265762No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)