FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007438413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007438413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49831184
Sequences flagged as poor quality0
Sequence length35-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3840250.7706519676514209No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA2952800.5925606744563805No Hit
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT2385210.47865810292607136No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG1570840.31523232520423355No Hit
CAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCTTAGCTGTGCTCGCGC1428280.2866237334437006No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT1377620.27645740867806795No Hit
AATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTG1316710.26423413900821624No Hit
GGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGT1171660.23512586014412182No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1126070.2259769705652589No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC1108000.22235072720728452No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCT1102160.22117877030575872No Hit
CCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTG1082200.21717324637520152No Hit
GGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCT1048480.21040639933420005No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1047080.2101254507619165No Hit
CACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTT1010830.20285088951528824No Hit
CACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCA1006730.20202811155360068No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT981770.1970191998648878No Hit
GAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATT860640.17271112803580987No Hit
CAGCAACTCACCCTCACTCAGAGGTCTTCTGGTTCTGGAAACAACTCTAG829070.16637573773081532No Hit
CTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAA763110.1531390464252264No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG722340.14495742264522554No Hit
CCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTC720050.14449787105199027No Hit
CCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT690640.13859594425851893No Hit
CAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAG680580.13657712808910982No Hit
GACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCTTAGCTGTGCTCGC677450.1359490073525044No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT674840.1354252389427472No Hit
GTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAG668940.13424124138812354No Hit
CTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAACCATGTC662770.13300306089455952No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT647510.12994072145666857No Hit
ATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAA576970.11578492696460915No Hit
GGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCG541660.10869900261651418No Hit
CTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTT539420.10824948490086048No Hit
CTTGTAGGCAGCAACTCACCCTCACTCAGAGGTCTTCTGGTTCTGGAAAC533650.10709157542794889No Hit
TGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAAT530040.10636712946656054No Hit
GTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCT528460.10605005893498337No Hit
CGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGG515530.10345529819239294No Hit
GTCTCGCTCCGTGGCCTTAGCTGTGCTCGCGCTACTCTCTCTTTCTGGCC509510.10224721933157357No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)