FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007438459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007438459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11428094
Sequences flagged as poor quality0
Sequence length87-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA219746919.228657027147307RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG118258210.348024788735549RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG7486746.551171175175844No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC6632415.80360119543994RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC2889602.5285056283226233RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA2776342.42939898814273RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA2764562.419091057528928No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA2567702.2468313613801216No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG2052241.7957850189191653Illumina Small RNA Adapter 2 (100% over 21bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1970361.7241370258242537RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1668601.460085995092445RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC1561411.3662908267992895RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA940820.8232518913477611RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA877270.767643318299622RNA PCR Primer, Index 1 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA842320.7370607907145321RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG833670.7294917245167917RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC796720.6971591238223978RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT782410.6846373507253266RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT695350.6084566682773173RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA691080.6047202621889529No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG677260.5926272570036614No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG648960.5678637225070078RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA628430.5498992220400007RNA PCR Primer, Index 1 (100% over 28bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG580940.5083437360595738No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG545790.4775862011635536RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAATGGAATTCTCGGGTGCCAAGGAACTCCA540620.4730622621760024RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT519360.45445898502409937RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC501000.43839331388068736RNA PCR Primer, Index 1 (100% over 26bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG499620.43718576343526755RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGAACCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG490230.4289691701870845RNA PCR Primer, Index 1 (100% over 29bp)
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA468020.40953460830826205RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA462710.4048881642030596RNA PCR Primer, Index 1 (100% over 23bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC438680.3838610357947703RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG417240.36510025206302993RNA PCR Primer, Index 1 (100% over 29bp)
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT401300.35115216938187593RNA PCR Primer, Index 1 (100% over 25bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA367710.3217596915111129RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA351130.30725158543498154RNA PCR Primer, Index 1 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA348070.3045739735777462RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT347930.3044514684600949RNA PCR Primer, Index 1 (100% over 30bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA340020.29752992931279704No Hit
CTAGACTGAAGCTCCTTGAGGATGGAATTCTCGGGTGCCAAGGAACTCCA338200.29593736278333027RNA PCR Primer, Index 1 (100% over 28bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA334520.2927172282622107No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC331270.2898733594595914RNA PCR Primer, Index 2 (100% over 50bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCC320640.2805717208836399No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC317130.27750034257681117RNA PCR Primer, Index 1 (100% over 26bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA298180.26091839986615445RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC292450.25590444040799804RNA PCR Primer, Index 1 (100% over 27bp)
TATGCATATGATCACGAGACTCAGTTGGAATTCTCGGGTGCCAAGGAACT292030.25553692505504416RNA PCR Primer, Index 1 (100% over 25bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA290180.2539181074289379RNA PCR Primer, Index 2 (100% over 36bp)
CGATGCTATAGACTCTCACGTGATGTGGAATTCTCGGGTGCCAAGGAACT284020.24852788225228106RNA PCR Primer, Index 1 (100% over 25bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA279000.24413519874792766RNA PCR Primer, Index 1 (100% over 28bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC274830.24048629631502855RNA PCR Primer, Index 2 (100% over 34bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA271360.23744991947038588RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC260250.22772826334820137RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGGGCAGAGAGCGAGACTTGGAATTCTCGGGTGCCAAGGAACTCCAG258960.2265994661927002RNA PCR Primer, Index 1 (100% over 29bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC247530.21659779837302703No Hit
TGAGGTAGTAGATTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC236500.20694614517521467RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG232860.20376101211628117RNA PCR Primer, Index 1 (100% over 29bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA231850.2028772251960826RNA PCR Primer, Index 1 (100% over 28bp)
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG221270.19361933844786366RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG219640.19219302886378078RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGATTGGAATTCTCGGGTGCCAAGGAACTCCA218980.1916155047377104RNA PCR Primer, Index 1 (100% over 28bp)
CTAGACTGAAGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG202460.17715990085485822RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC192670.16859329298481446RNA PCR Primer, Index 1 (100% over 26bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG172720.15113631371950562RNA PCR Primer, Index 2 (100% over 35bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA171010.1496400012110506RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATTGGAATTCTCGGGTGCCAAGGAACTC167880.14690113679498962RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGAGAATGGAATTCTCGGGTGCCAAGGAACTC166420.14562358342519757RNA PCR Primer, Index 1 (100% over 26bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC163890.14340974094192785RNA PCR Primer, Index 1 (100% over 27bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA161630.1414321583284142RNA PCR Primer, Index 1 (100% over 28bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC157750.13803701649636413RNA PCR Primer, Index 1 (100% over 33bp)
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA150740.13190301024825313RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCGCGTATGC144960.12684529896236416RNA PCR Primer, Index 2 (98% over 50bp)
AGCAGCATTGTACAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA144940.1268277982312711RNA PCR Primer, Index 1 (100% over 32bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC141710.12400143015974492No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA140790.12319639652946501RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC139720.12226010741598729RNA PCR Primer, Index 1 (100% over 26bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG137680.12047503284449708RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC130970.11460353756278169RNA PCR Primer, Index 1 (100% over 27bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA126090.11033335917607957RNA PCR Primer, Index 1 (100% over 28bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA123370.10795325974742595RNA PCR Primer, Index 1 (100% over 32bp)
TCCTGTACTGAGCTGCCCCGAGTTTGGAATTCTCGGGTGCCAAGGAACTC123310.10790075755414683RNA PCR Primer, Index 1 (100% over 26bp)
TACCCTGTAGATCCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG121820.10659695308771523RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA121590.10639569468014526No Hit
CATTGCACTTGTCTCGGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT115510.10107547242786066RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)