FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007438475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007438475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15840831
Sequences flagged as poor quality0
Sequence length88-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA551004734.783825419259884RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG402261925.3939897471288RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC10745626.7834951335570715RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC3388252.138934504130497RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2323571.4668232998634982RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT2231691.4088212922668009RNA PCR Primer, Index 1 (100% over 30bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1965611.2408503064012235RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA1892481.1946847990487368RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTTGGAATTCTCGGGTGCCAAGGAACTCCAG1504550.9497923435961156RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1228770.7756979416041999RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1123340.7091420898310197RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG1119050.7064338985751442RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC900570.5685118413295364RNA PCR Primer, Index 1 (100% over 27bp)
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG776460.490163678913057RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC762000.48103536992472173RNA PCR Primer, Index 1 (100% over 27bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT690770.4360692945969817RNA PCR Primer, Index 1 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGAATGGAATTCTCGGGTGCCAAGGAACTCCA601980.3800179422405302RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG583250.36819406759658RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG562750.3552528273295763RNA PCR Primer, Index 1 (100% over 29bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA476510.3008112390063375RNA PCR Primer, Index 1 (100% over 28bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG454570.28696095552057843RNA PCR Primer, Index 1 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA445580.28128574820348756RNA PCR Primer, Index 1 (100% over 28bp)
CATTGCACTTGTCTCGGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT427020.26956919116175154RNA PCR Primer, Index 1 (100% over 30bp)
TACCCTGTAGAACCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG425240.2684455127385678RNA PCR Primer, Index 1 (100% over 29bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG416910.26318695023007316RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCATTGTACAGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC407780.2574233637111588RNA PCR Primer, Index 1 (100% over 31bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC374010.23610503767131913RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA339890.21456576362692084RNA PCR Primer, Index 1 (100% over 28bp)
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA336880.21266561078771687RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG328730.20752067868156665No Hit
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT328550.20740704827922224RNA PCR Primer, Index 1 (100% over 25bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA328150.20715453627401242RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTTTGGAATTCTCGGGTGCCAAGGAACTCCA323820.204421093817616RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA295180.18634123424459234RNA PCR Primer, Index 1 (100% over 28bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT278000.17549584362083023RNA PCR Primer, Index 3 (100% over 34bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT276880.17478881000624272RNA PCR Primer, Index 1 (100% over 30bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG264840.1671881986494269RNA PCR Primer, Index 7 (97% over 36bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT262700.16583725942155433RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC258390.16311644256541843RNA PCR Primer, Index 1 (100% over 26bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA251960.15905731208167045RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG240120.15158295672745956RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA234690.14815510625673614RNA PCR Primer, Index 1 (100% over 28bp)
TATGCATATGATCACGAGACTCAGTTGGAATTCTCGGGTGCCAAGGAACT233160.14718924783680856RNA PCR Primer, Index 1 (100% over 25bp)
TCCTGTACTGAGCTGCCCCGAGAATGGAATTCTCGGGTGCCAAGGAACTC223870.14132465651581033RNA PCR Primer, Index 1 (100% over 26bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA222240.14029567009458027RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA217800.13749278683675117RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGC214010.13510023558738804RNA PCR Primer, Index 10 (100% over 50bp)
TAGCAGCACATAATGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT206130.13012574908475447RNA PCR Primer, Index 1 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGAGATTGGAATTCTCGGGTGCCAAGGAACTC203520.1284781082507603RNA PCR Primer, Index 1 (100% over 26bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG198530.12532802098576773RNA PCR Primer, Index 1 (100% over 29bp)
CGATGCTATAGACTCTCACGTGATGTGGAATTCTCGGGTGCCAAGGAACT197360.12458942337052899RNA PCR Primer, Index 1 (100% over 25bp)
TCCTGTACTGAGCTGCCCCGATTGGAATTCTCGGGTGCCAAGGAACTCCA193730.1222978769232498RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGGTGGAATTCTCGGGTGCCAAGGAACTCCAG181130.11434374875914023RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCATTGTACAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA179260.11316325513478429RNA PCR Primer, Index 1 (100% over 32bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC178720.11282236392775101RNA PCR Primer, Index 1 (100% over 26bp)
TGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT170300.10750698621808413RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC165580.10452734455660817RNA PCR Primer, Index 1 (100% over 26bp)
CTAGACTGAAGCTCCTTGAGGATGGAATTCTCGGGTGCCAAGGAACTCCA165240.10431270935217982RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)