FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007438488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007438488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9375178
Sequences flagged as poor quality0
Sequence length92-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA107853011.504101575458087RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG6877397.335743385352258RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG6727407.1757570896253915No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA5065295.402873417443381RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC3202973.4164364665929545RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA2328092.4832488513818083No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1890932.0169537047723254RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG1762201.8796443118199995Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC1562381.6665070252532805RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC1442751.538904114673876RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1394411.4873424269917863RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1203881.2841142856167638RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1122761.1975879284638649No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG968481.0330257196183368RNA PCR Primer, Index 1 (100% over 29bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG806290.8600263376332694No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC752760.8029287550593706RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA695440.7417885825741122RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA681020.7264075412754829RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT654920.698568069854247RNA PCR Primer, Index 1 (100% over 30bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA603850.6440944374602807RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA555050.5920420924274717RNA PCR Primer, Index 1 (100% over 23bp)
TACCCTGTAGAACCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG530150.5654825967037639RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACGGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG509850.5438296744872471RNA PCR Primer, Index 1 (100% over 29bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT500030.5333552066958088RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA497160.5302939314858875No Hit
TCCTGTACTGAGCTGCCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT494980.5279686423020448RNA PCR Primer, Index 1 (100% over 30bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG492670.5255046890843033RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC454270.4845454667634043RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA452760.4829348306773482RNA PCR Primer, Index 1 (100% over 28bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA444430.47404966604367405No Hit
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT433270.46214589205666284RNA PCR Primer, Index 1 (100% over 25bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC431880.4606632535403594RNA PCR Primer, Index 1 (100% over 26bp)
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA429340.4579539716472583RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA428710.45728198440605605RNA PCR Primer, Index 1 (100% over 28bp)
TATGCATATGATCACGAGACTCAGTTGGAATTCTCGGGTGCCAAGGAACT402670.42950651176969656RNA PCR Primer, Index 1 (100% over 25bp)
TGAGGTAGTAGATTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC378970.4042269917435168RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC377880.40306434715159545RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT366160.3905632511724044RNA PCR Primer, Index 1 (100% over 30bp)
CGATGCTATAGACTCTCACGTGATGTGGAATTCTCGGGTGCCAAGGAACT331670.3537746163326179RNA PCR Primer, Index 1 (100% over 25bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA326160.34789739458813473RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG313460.3343509851226291RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA297350.31716731138331455RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC295800.3155140094406741RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGAATGGAATTCTCGGGTGCCAAGGAACTCCA295310.31499135269751677RNA PCR Primer, Index 1 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA288120.3073221649764943RNA PCR Primer, Index 1 (100% over 28bp)
CTAGACTGAAGCTCCTTGAGGATGGAATTCTCGGGTGCCAAGGAACTCCA282920.3017756036205393RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG247980.2645069778941797RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG245650.262021691748146No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGTATGC241130.25720044995412356RNA PCR Primer, Index 15 (100% over 50bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA237260.2530725283295955RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG233540.24910460366725837RNA PCR Primer, Index 1 (100% over 29bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA223640.2385448041626516No Hit
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG222850.23770215349511228RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC216330.23074761887187634RNA PCR Primer, Index 1 (100% over 27bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA215310.22965963952897744RNA PCR Primer, Index 1 (100% over 34bp)
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG215280.22962764013653927RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGGGCAGAGAGCGAGACTTGGAATTCTCGGGTGCCAAGGAACTCCAG213970.22823033333340442RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC207930.22178778898917975RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC200890.21427859823034828RNA PCR Primer, Index 1 (100% over 27bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA193950.20687607211297745RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC183100.19530295851449433RNA PCR Primer, Index 1 (100% over 27bp)
CTAGACTGAAGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG176980.18877508245710106RNA PCR Primer, Index 1 (100% over 29bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC176320.18807109582346063No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCC174630.18626846338277522No Hit
AGCAGCATTGTACAGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC170280.1816285514792359RNA PCR Primer, Index 1 (100% over 31bp)
AGCAGCATTGTACAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA169150.1804232410307303RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA165680.1767219779720449RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG161500.17226339595898874RNA PCR Primer, Index 15 (100% over 36bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC159330.1699487732392921RNA PCR Primer, Index 1 (100% over 26bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA149190.15913297859517975RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA137680.14685587836305614RNA PCR Primer, Index 1 (100% over 28bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT136620.145725233163573RNA PCR Primer, Index 1 (100% over 35bp)
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG134900.1438906013304494RNA PCR Primer, Index 1 (100% over 29bp)
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA133970.14289862016486513RNA PCR Primer, Index 1 (100% over 28bp)
CATTGCACTTGTCTCGGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT126950.1354107623343258RNA PCR Primer, Index 1 (100% over 30bp)
TACCCTGTAGATCCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG125590.13396012321046066RNA PCR Primer, Index 1 (100% over 29bp)
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACTC122800.13098417971370782RNA PCR Primer, Index 1 (100% over 26bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG122100.1302375272234831RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGATTGGAATTCTCGGGTGCCAAGGAACTCCA117890.12574694581798873RNA PCR Primer, Index 1 (100% over 28bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC116270.12401897862632581RNA PCR Primer, Index 1 (100% over 33bp)
TTTGGCAATGGTAGAACTCACATGGAATTCTCGGGTGCCAAGGAACTCCA115540.1232403267436629RNA PCR Primer, Index 1 (100% over 28bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC112680.12018971799788762RNA PCR Primer, Index 1 (100% over 26bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC109270.11655245372407862RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGAGAATGGAATTCTCGGGTGCCAAGGAACTC108950.11621112687140447RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC108740.11598713112433705RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT108660.11590179941116852RNA PCR Primer, Index 1 (100% over 30bp)
TGTCAGTTTGTCAAATACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC107300.11445116028730333RNA PCR Primer, Index 1 (100% over 31bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC105210.11222186928077525No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA104490.11145388386225841RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA103220.11009924291570784RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA101000.10773128787528088No Hit
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTC97510.10400869188830335RNA PCR Primer, Index 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)