FastQCFastQC Report
Fri 13 Jan 2023
EGAF00007449595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007449595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101543165
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3150630.31027494563518876TruSeq Adapter, Index 25 (97% over 44bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA2767070.27250184687467643TruSeq Adapter, Index 25 (97% over 43bp)
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGT1222540.12039608968264874No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)