FastQCFastQC Report
Fri 3 May 2024
EGAF00007449712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007449712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74687426
Total Bases5.6 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT1567220.20983719535333833No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT1508350.20195501181149286No Hit
GGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTATG1270020.1700446873078743No Hit
GGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGG1165130.15600082402090013No Hit
AGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAATCCTGCGAAGAAAA1137230.15226525546616107No Hit
GGGCAGTGAGAGTGAGTAGTAGAATGTTTAGTGAGCCTAGGGTGTTGTGA1064870.14257687766612817No Hit
GGGAGGGCGATGAGGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGT850390.11385986176575424No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC812460.10878136301015381TruSeq Adapter, Index 1 (100% over 50bp)
GGGTGGTTGGTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTG792150.10606202977191904No Hit
GGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAG786740.10533767758979938No Hit
GCGGTCTGTTAGTAGTATAGTGATGCCAGCAGCTAGGACTGGGAGAGATA768230.102859348774451No Hit
GGCTGGAGTGGTAAAAGGCTCAGAAAAATCCTGCGAAGAAAAAAACTTCT767740.10279374201488749No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)