FastQCFastQC Report
Fri 13 Jan 2023
EGAF00007449742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007449742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177082096
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGT9267130.5233239389712215No Hit
GTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAG5838810.32972333916806584No Hit
GTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTC5797860.3274108524218055No Hit
GGGAGATCATAATATTGTTGACAGAAATATGTTGCAATATCTTCAGGCTGCAGGCCGCTGATGGTTAAAAAAAAA5108580.2884865333873166No Hit
GGGGAGATCATAATATTGTTGACAGAAATATGTTGCAATATCTTCAGGCTGCAGGCCGCTGATGGTTAAAAAAAA4874600.2752734528283424No Hit
CCCGGGTCTCTGCTGATACCAATTTAAATAGTTTCTGATGTCCTGACTCGCCTGGCAAGTGATAGTGACTCTGTC4693430.2650426048718104No Hit
GTCCTGACTCGCCTGGCAAGTGATAGTGACTCTGTCTCCTATAGATGTAGACAGGGAGGATGGAGACTGGGTCAT4048590.22862785631360497No Hit
ATCGAAGATCAGGAGTTTAGGGGGTTTCCCGGGTCTCTGCTGATACCAATTTAAATAGTTTCTGATGTCCTGACT3724970.21035271685512463No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTC3683110.20798884151450298No Hit
CCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCT3505680.1979691950336978No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTT3270150.18466858445136092No Hit
CTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAG3238630.18288861907304282No Hit
GGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTA3120230.17620245470778706No Hit
GTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT2985180.1685760484786672No Hit
CTCTGCTGATACCAATTTAAATAGTTTCTGATGTCCTGACTCGCCTGGCAAGTGATAGTGACTCTGTCTCCTATA2862920.16167190611974686No Hit
GCTCATTGTAAGGACCACTTGGTTTTTGGAGGTGTCCTTGGAGATGGTGAGCCTGGTCTTCAGAGATGTACTGTA2775750.15674933054779294No Hit
GTTGGACATCCACTTTGGTCCCAGGGCCGAAAGTGAGGGGGAGATCATAATATTGTTGACAGAAATATGTTGCAA2723220.15378290982053883No Hit
TGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTG2693930.15212887473389744No Hit
GCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGA2689060.15185386104759002No Hit
CTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGAC2627280.14836508372930032No Hit
GTAGAATTTATCATCATCCCAATCGATGAGTGCAAGCCACTCCAGGGCCTTCCCTGGGGGCTGACGGATCCAGGT2470530.13951325717310234No Hit
GTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTC2433350.1374136660320533No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2384160.13463585838740016TruSeq Adapter, Index 22 (97% over 40bp)
CTGCTGATACCAATTTAAATAGTTTCTGATGTCCTGACTCGCCTGGCAAGTGATAGTGACTCTGTCTCCTATAGA2329550.13155197801589155No Hit
GTGGCGGTTAGCGGGGTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCG2280200.1287651350139881No Hit
CGCTGATGGTTAAAAAAAAATCTGTCCCAGATCCACTTCCACTGAACCTTGATGGGACCCCTGTCTCCAAATTGG2194880.12394703076024129No Hit
CGAAGATCAGGAGTTTAGGGGGTTTCCCGGGTCTCTGCTGATACCAATTTAAATAGTTTCTGATGTCCTGACTCG2175240.12283794065776135No Hit
CCTGTCTCCAAATTGGATGCATCGAAGATCAGGAGTTTAGGGGGTTTCCCGGGTCTCTGCTGATACCAATTTAAA2160150.12198579352708813No Hit
GTTGCAATATCTTCAGGCTGCAGGCCGCTGATGGTTAAAAAAAAATCTGTCCCAGATCCACTTCCACTGAACCTT2072830.11705474730771201No Hit
GTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACG2051560.11585360950324419No Hit
GGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGG2044370.11544758313680678No Hit
CCGCTGATGGTTAAAAAAAAATCTGTCCCAGATCCACTTCCACTGAACCTTGATGGGACCCCTGTCTCCAAATTG2003540.11314187290848421No Hit
GTGACGTAGTCGCTCGCCCCCTGTCCACCCGTGCACAGAAGTACGTGGCTGTGTCTCCTGGGGCCATGTTGCTCA1971390.11132633081099289No Hit
GCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTGGACATCCACTTTGGTCCCAGGGCCGAAAGTGAGGG1965260.11098016368633902No Hit
CTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCT1951280.11019069934659007No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCAC1857580.10489936825685642No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG1798650.10157153323958848No Hit
GTTAAAAAAAAATCTGTCCCAGATCCACTTCCACTGAACCTTGATGGGACCCCTGTCTCCAAATTGGATGCATCG1771580.10004286373479564No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)