FastQCFastQC Report
Thu 5 Jan 2023
EGAF00007449800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007449800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85758480
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1748270.20385972326002047TruSeq Adapter, Index 7 (97% over 35bp)
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACGAGCTCAACAAGTGCAGTTTGTTTCTTGATTTGTCTCTCCTTCTC1648890.19227136488426566No Hit
GGCGTTTTCTCATGCAACACACATAACTGGTTCAGGACCACGGATAGATAGTCTTCTGCACAGGGCATTCTTTTT1417820.16532709068537596No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATCATCCATAAC1386370.1616598148661217No Hit
CCGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTTTCAAAAATGTCTCTTCATTGTCAT1251750.1459622418680928No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTTGCAGCAACAAGTTTTTTACCCTCCT1202790.14025318545757806No Hit
GGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTTTCAAAAATGTCTCTTCATTGTCATGA969250.11302089309418731No Hit
GTGGGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCC873270.1018289969691627No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)