FastQCFastQC Report
Thu 5 Jan 2023
EGAF00007449804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007449804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61609306
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA9907371.6080963483016673TruSeq Adapter, Index 1 (100% over 63bp)
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGCATGACCTCACGCCTTCTATAAAA1000290.16236021227052938No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGCGTTTAGAGTGCAATTTTGTCAGCAG959090.15567291084239773No Hit
AGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTGGATACCACTTTGAGGCATGTAATAT873280.14174482017375753No Hit
AGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCAAGGGTTCTGCACAACTTATGCACCT870330.141265996406452No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATTGTTAACTCCGAGCTAGACTTCAGGT800640.12995439357813898No Hit
AGTATGGCCCGGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGACACACAGGACCCAGGCGTGCAAGTC712140.11558968055897269No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGCATGACCTCACGC705160.11445673483158533No Hit
GGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCAAGGGTTCTGCACAACTTATGCACCTCTATTAGATC681180.11056446569938638No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCACGCCCATATAAACGATCGGATAAATAGCTGAATTTCCATGC644560.10462055845913928No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)