FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007449812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007449812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71493633
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3522300.49267324266483986TruSeq Adapter, Index 27 (97% over 44bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1668870.23342917823185738TruSeq Adapter, Index 27 (97% over 43bp)
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGCGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGT1250700.17493865502680497No Hit
GGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTTCAGTACCATTGGTGGCCA899840.1258629562159752No Hit
GGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTATTAGTGGGAGTAGAGTTTGAAGTCC822440.11503681733448906No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)