FastQCFastQC Report
Fri 13 Jan 2023
EGAF00007449844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007449844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67274346
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA33729935.013787870936716TruSeq Adapter, Index 1 (100% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA7408871.1012920140464837TruSeq Adapter, Index 1 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC912190.13559254816092897TruSeq Adapter, Index 1 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA680110.10109499986815182TruSeq Adapter, Index 1 (98% over 62bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)