FastQCFastQC Report
Fri 13 Jan 2023
EGAF00007449846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007449846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85124513
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4764840.5597494578324342TruSeq Adapter, Index 7 (97% over 35bp)
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACGAGCTCAACAAGTGCAGTTTGTTTCTTGATTTGTCTCTCCTTCTC1010050.11865559806491933No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATCATCCATAAC881040.10350015159558093No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)