FastQCFastQC Report
Thu 16 May 2024
EGAF00007456986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007456986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70951567
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT6491210.914879018810113TruSeq Adapter, Index 25 (97% over 44bp)
CCGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTT1631230.22990753678491693No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT1493200.21045342099350675No Hit
GGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTTT1389310.19581103825374288No Hit
CGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTT1313780.18516574834774263No Hit
GTGGGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGT1193590.16822602381706384No Hit
GGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTCTCAGGGT972090.13700754487917088No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT846740.11934056368339266No Hit
GTGGCAATAAAAATGATTAAGGATACTAGTATAAGAGATCAGGTTCGTCC749550.10564248707854472No Hit
GGGACGATTAGTTTTAGCATTGGAGTAGGTTTAGGTTATGTACGTAGTCT739150.1041766984512125No Hit
CTCCATTTCTATCAAAAGTTCTGTGGGTCCCATCCTGGTAAGCTGGCTAA719610.10142270712639792No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG719360.10138747182285629No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)