FastQCFastQC Report
Fri 13 Jan 2023
EGAF00007456986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007456986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70951567
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTTTCAAAAATGTCTCTTCATTGTCAT1600090.22551862737577028No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG1531190.21580777771969434TruSeq Adapter, Index 25 (97% over 44bp)
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGT1467480.20682841296514282No Hit
GGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTTTCAAAAATGTCTCTTCATTGTCATGA1366810.19263986093499527No Hit
CGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTTTCAAAAATGTCTCTTCATTGTCATG1290470.1818804086455201No Hit
GTGGGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCC1172740.16528739950169108No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAGG1065010.1501038024995276TruSeq Adapter, Index 25 (97% over 44bp)
GGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTCTCAGGGTTTGTTATAATTTTTTATTTTTATGG949360.13380395108116497No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAGCCTGTGGCTACAAAAA835790.11779725738826881No Hit
GTGGCAATAAAAATGATTAAGGATACTAGTATAAGAGATCAGGTTCGTCCTTTAGTGTTGTGTATGGTTATCATT719810.10145089536923123No Hit
GGGACGATTAGTTTTAGCATTGGAGTAGGTTTAGGTTATGTACGTAGTCTAGGCCATATGTGTTGGAGATTGAGA719130.10135505534359798No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)