FastQCFastQC Report
Fri 13 Jan 2023
EGAF00007457026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007457026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79960570
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC2449590.3063497421291519No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAGAATCTCGGTT1520760.1901887392748701TruSeq Adapter, Index 20 (97% over 37bp)
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC1487250.18599792372665677No Hit
CTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCA1456080.18209975241547177No Hit
CCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTG1253370.15674850742059493No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC1216530.15214123661199513No Hit
CCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATA1215520.15201492435584188No Hit
CTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTT1066270.13334947462230448No Hit
CCCTTCTGCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGAC1048000.13106459846396792No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAGAATCTCGTTT990820.12391357390273731TruSeq Adapter, Index 20 (97% over 37bp)
CCCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT960310.12009794327379106No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG914230.11433510291384867No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA897800.1122803401726626No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAGAATCGCGGTT874260.10933638917281355TruSeq Adapter, Index 20 (97% over 37bp)
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC853740.10677012432502671No Hit
GGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCA818660.10238296200239694No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT816930.10216660536561958No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)