FastQCFastQC Report
Fri 13 Jan 2023
EGAF00007457036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007457036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88495586
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA10286911.1624206884171602TruSeq Adapter, Index 7 (97% over 36bp)
GTGGGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCC1604870.18135028791153493No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGT1322230.14941197180162186No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAT1186530.13407787366931498TruSeq Adapter, Index 7 (97% over 36bp)
GGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTATGTAAAGGATGCGTAGGGATGGGAGGG964230.10895797672891842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)