FastQCFastQC Report
Fri 2 Jun 2023
EGAF00007457238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007457238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92523029
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA9255051.0002969098644618TruSeq Adapter, Index 20 (97% over 44bp)
GTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGT1026650.11096156395830924No Hit
CTAGATTTTATGTATACGGGTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTATG1015650.10977267075854164No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGA939720.10156606524414588No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)