FastQCFastQC Report
Fri 13 Jan 2023
EGAF00007457346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007457346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73189275
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCACAGCAATCTCGTAT9081311.2407979174544357TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCACAGCAATCGCGTAT3683160.503237666994242TruSeq Adapter, Index 4 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2219160.3032083594215136No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCACAGCAATCTCGGAT815850.11147125039836778TruSeq Adapter, Index 4 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)