FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007543392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80070431
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAGCTCAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT34284324.281770382877045Illumina Single End PCR Primer 1 (100% over 39bp)
ACAACCGGTTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT28582763.5697022787350803Illumina Single End PCR Primer 1 (100% over 39bp)
GCATTCGAATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT7069320.8828877166903223Illumina Single End PCR Primer 1 (100% over 39bp)
CAAGAGCTCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT6865250.8574014045209774Illumina Single End PCR Primer 1 (100% over 39bp)
TGCTGCGCAGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT6722970.839632048439954Illumina Single End PCR Primer 1 (100% over 39bp)
CCATAGCTATGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT6354080.793561358499494Illumina Single End PCR Primer 1 (100% over 39bp)
TATCTCGAGATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT5637190.7040289317288675Illumina Single End PCR Primer 1 (100% over 39bp)
CTATTGCAATAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT4349940.5432642169741786Illumina Single End PCR Primer 1 (100% over 39bp)
CATGACGTCATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT3668520.45816164021897177Illumina Single End PCR Primer 1 (100% over 39bp)
CTCAGCGCTGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT3420660.4272063928318308Illumina Single End PCR Primer 1 (100% over 39bp)
ATCGACGTCGATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT3011360.3760888960370402Illumina Single End PCR Primer 1 (100% over 39bp)
GTGAGGCCTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT2394550.2990554653065374Illumina Single End PCR Primer 1 (100% over 39bp)
ACTCACGTGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT2355720.29420598472861975Illumina Single End PCR Primer 1 (100% over 39bp)
AGGATGCATCCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT2337710.2919567149576102Illumina Single End PCR Primer 1 (100% over 39bp)
GAAGTCGACTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1601000.1999489674284381Illumina Single End PCR Primer 1 (100% over 39bp)
ATTGAGCTCAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1507400.18825925890170367Illumina Single End PCR Primer 1 (100% over 39bp)
GTTAAGCTCAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1394840.17420163505801536Illumina Single End PCR Primer 1 (100% over 39bp)
ACAGACGTCTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1324070.1653631663353979Illumina Single End PCR Primer 1 (100% over 39bp)
CGGTAGCTACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1155990.14437164700662095Illumina Single End PCR Primer 1 (100% over 39bp)
TGTAGGCCTACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1085040.13551069807529822Illumina Single End PCR Primer 1 (100% over 39bp)
ACAACCGGTTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT920990.11502248564142237Illumina Single End PCR Primer 1 (100% over 39bp)
TGACAGCTGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT845780.10562950510407518Illumina Single End PCR Primer 1 (100% over 39bp)
ACAACCGGTTGTAAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT824960.10302929429716694Illumina Single End PCR Primer 1 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)