FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007543395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103715556
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAGCTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTC7243010.698353292345075TruSeq Adapter, Index 1 (97% over 36bp)
ACAACCGGTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTC6302950.6077150085373886TruSeq Adapter, Index 1 (97% over 36bp)
CCATAGCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTC6036190.5819946624014627TruSeq Adapter, Index 1 (97% over 36bp)
TGCTGCGCAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTC5359740.5167730094413223TruSeq Adapter, Index 1 (97% over 36bp)
CAAGAGCTCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTC3857950.3719740942236283TruSeq Adapter, Index 1 (97% over 36bp)
CTATTGCAATAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTC3566810.34390308817319554TruSeq Adapter, Index 1 (97% over 36bp)
GCATTCGAATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTC2418690.2332041685241508TruSeq Adapter, Index 1 (97% over 36bp)
TATCTCGAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTC1584600.1527832526877646TruSeq Adapter, Index 1 (97% over 36bp)
GTGAGGCCTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTC1568670.15124732108653016TruSeq Adapter, Index 1 (97% over 36bp)
AGAAGGTCCTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCT1118950.10788641966109694TruSeq Adapter, Index 1 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)