FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007543398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90584664
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGCGCAGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT26477202.922923023702997Illumina Single End PCR Primer 1 (100% over 39bp)
CCATAGCTATGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT17542291.9365629042902892Illumina Single End PCR Primer 1 (100% over 39bp)
GTTGAGCTCAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT8100860.8942860349959459Illumina Single End PCR Primer 1 (100% over 39bp)
ACAACCGGTTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT5807240.6410842347441946Illumina Single End PCR Primer 1 (100% over 39bp)
CTATTGCAATAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT4648180.5131310085777875Illumina Single End PCR Primer 1 (100% over 39bp)
CAAGAGCTCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT4118640.4546729896795776Illumina Single End PCR Primer 1 (100% over 39bp)
GAAGTCGACTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT3003330.3315494993722116Illumina Single End PCR Primer 1 (100% over 39bp)
TATCTCGAGATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT2884560.31843800844699277Illumina Single End PCR Primer 1 (100% over 39bp)
GCATTCGAATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT2273220.2509497634169069Illumina Single End PCR Primer 1 (100% over 39bp)
CTCAGCGCTGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1795710.19823554238717492Illumina Single End PCR Primer 1 (100% over 39bp)
GTGAGGCCTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1746310.1927820806400518Illumina Single End PCR Primer 1 (100% over 39bp)
TCTCTGCAGAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1651880.18235757876189726Illumina Single End PCR Primer 1 (100% over 39bp)
ATCGACGTCGATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1360830.15022741597849276Illumina Single End PCR Primer 1 (100% over 39bp)
TGTAGGCCTACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1316600.14534469101745523Illumina Single End PCR Primer 1 (100% over 39bp)
TGCTGCGCAACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1112140.12277354144626512Illumina Single End PCR Primer 1 (100% over 39bp)
AGAAGGCCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1104850.12196876945969573Illumina Single End PCR Primer 1 (100% over 39bp)
TGACAGCTGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1041290.11495212920368066Illumina Single End PCR Primer 1 (100% over 39bp)
ACAGACGTCTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1022800.11291094483719673Illumina Single End PCR Primer 1 (100% over 39bp)
CCATAGCTATGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1022280.1128535399767007Illumina Single End PCR Primer 1 (100% over 39bp)
AGGATGCATCCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT978900.10806464988378166Illumina Single End PCR Primer 1 (100% over 39bp)
TACTGCGCAGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT940070.10377805232020292Illumina Single End PCR Primer 1 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)