FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007543399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86770244
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAGCTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT23202382.674001930892346TruSeq Adapter, Index 4 (97% over 37bp)
CAAGAGCTCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT15388911.773523882219347TruSeq Adapter, Index 4 (97% over 37bp)
ACAACCGGTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT13433381.5481551486705512TruSeq Adapter, Index 4 (97% over 37bp)
GCATTCGAATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT7097490.8179635866876207TruSeq Adapter, Index 4 (97% over 37bp)
TATCTCGAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT6763100.7794261820907177TruSeq Adapter, Index 4 (97% over 37bp)
CCATAGCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT6265420.7220701142663607TruSeq Adapter, Index 4 (97% over 37bp)
CTCAGCGCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT5724000.6596731478593053TruSeq Adapter, Index 4 (97% over 37bp)
CTATTGCAATAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT4947160.5701447606854718TruSeq Adapter, Index 4 (97% over 37bp)
TGCTGCGCAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT4245680.48930137847716554TruSeq Adapter, Index 4 (97% over 37bp)
ATCGACGTCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT2669630.30766653139756067TruSeq Adapter, Index 4 (97% over 37bp)
GAAGTCGACTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT2364970.27255541657806104TruSeq Adapter, Index 4 (97% over 37bp)
TGACAGCTGTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT2068470.23838471630896876TruSeq Adapter, Index 4 (97% over 37bp)
GTGAGGCCTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT1742620.2008315200773205TruSeq Adapter, Index 4 (97% over 37bp)
AGAAGGCCTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT1681550.19379339304381812TruSeq Adapter, Index 4 (97% over 37bp)
ACTCACGTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT1613840.18599002671929793TruSeq Adapter, Index 4 (97% over 37bp)
AGGATGCATCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT1385580.15968377362174987TruSeq Adapter, Index 4 (97% over 37bp)
GTTGAGCTCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT1126730.12985211842898586TruSeq Adapter, Index 4 (97% over 37bp)
GTGAGGTCCTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAC1050300.12104379930059894TruSeq Adapter, Index 4 (97% over 37bp)
TGTAGGTCCTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAC1022190.11780420947070289TruSeq Adapter, Index 4 (97% over 37bp)
GTTGAGCTTAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT964160.1111164329559797TruSeq Adapter, Index 4 (97% over 37bp)
AGAAGGTTGTAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA902020.1039549917596175No Hit
TATCTCGATCGGAAGAGCACACGTCTGAACGAGATAAGATCGGAAGAGCAC899440.10365765480617986Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
CGGTAGCTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT894300.10306528583692814TruSeq Adapter, Index 4 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)