FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007543400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86770244
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAGCTCAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT23675712.728551737160034Illumina Single End PCR Primer 1 (100% over 39bp)
CAAGAGCTCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT15073751.737202675147485Illumina Single End PCR Primer 1 (100% over 39bp)
ACAACCGGTTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT13079241.507341618170395Illumina Single End PCR Primer 1 (100% over 39bp)
GCATTCGAATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT6937840.7995644221076524Illumina Single End PCR Primer 1 (100% over 39bp)
TATCTCGAGATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT6654080.76686196710476Illumina Single End PCR Primer 1 (100% over 39bp)
CCATAGCTATGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT6070780.6996384613139961Illumina Single End PCR Primer 1 (100% over 39bp)
CTCAGCGCTGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT5773680.6653986129161974Illumina Single End PCR Primer 1 (100% over 39bp)
CTATTGCAATAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT4843770.5582293856405429Illumina Single End PCR Primer 1 (100% over 39bp)
TGCTGCGCAGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT4337810.4999190736400373Illumina Single End PCR Primer 1 (100% over 39bp)
ATCGACGTCGATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT2616710.3015676664456539Illumina Single End PCR Primer 1 (100% over 39bp)
GAAGTCGACTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT2335030.2691049249556104Illumina Single End PCR Primer 1 (100% over 39bp)
TGACAGCTGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT2047110.23592304292702002Illumina Single End PCR Primer 1 (100% over 39bp)
GTGAGGCCTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1774180.20446871164727853Illumina Single End PCR Primer 1 (100% over 39bp)
AGAAGGCCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1622490.18698691224148223Illumina Single End PCR Primer 1 (100% over 39bp)
ACTCACGTGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1555520.17926882860903331Illumina Single End PCR Primer 1 (100% over 39bp)
AGGATGCATCCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1352600.15588293148052Illumina Single End PCR Primer 1 (100% over 39bp)
ATTGAGCTCAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1098040.12654568540800692Illumina Single End PCR Primer 1 (100% over 39bp)
GTGAGGACCTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1012230.11665635053417622Illumina Single End PCR Primer 1 (100% over 38bp)
TGTAGGACCTACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1008490.11622532719857283Illumina Single End PCR Primer 1 (100% over 38bp)
GTTAAGCTCAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT963220.11100810088767296Illumina Single End PCR Primer 1 (100% over 39bp)
TGTAGGCCTACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT874270.10075689080694529Illumina Single End PCR Primer 1 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)