FastQCFastQC Report
Wed 28 Dec 2022
EGAF00007543409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21943
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAGCTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG3141.430980267055553TruSeq Adapter, Index 2 (97% over 38bp)
ACAACCGGTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG2951.3443922891127011TruSeq Adapter, Index 2 (97% over 38bp)
TGCTGCGCAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG1740.7929635874766441TruSeq Adapter, Index 2 (97% over 38bp)
CTATTGCAATAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG1210.5514287016360571TruSeq Adapter, Index 2 (97% over 38bp)
CAAGAGCTCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG1170.5331996536480883TruSeq Adapter, Index 2 (97% over 38bp)
CCATAGCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG930.42382536572027524TruSeq Adapter, Index 2 (97% over 38bp)
TATCTCGAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG780.35546643576539216TruSeq Adapter, Index 2 (97% over 38bp)
TGTAGGCCTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG720.3281228637834389TruSeq Adapter, Index 2 (97% over 38bp)
GTGAGGCCTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG690.3144510777924623TruSeq Adapter, Index 2 (97% over 38bp)
AGGATGCATCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG460.20963405186164152TruSeq Adapter, Index 2 (97% over 38bp)
CTCAGCGCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG450.20507678986464933TruSeq Adapter, Index 2 (97% over 38bp)
GAAGTCGACTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG390.17773321788269608TruSeq Adapter, Index 2 (97% over 38bp)
GCATTCGAATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG280.1276033359157818TruSeq Adapter, Index 2 (97% over 38bp)
CATGACGTCATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG260.11848881192179739TruSeq Adapter, Index 2 (97% over 38bp)
GTTGAGCTTAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG260.11848881192179739TruSeq Adapter, Index 2 (97% over 38bp)
TCTCTGCAGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG220.10025976393382854TruSeq Adapter, Index 2 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)